MECA-79 anticorps (Biotin)

N° du produit ABIN7656727

Cet anticorps Rat Monoclonal détecte spécifiquement dans ELISA, FACS, IF, IHC, Func et IHC (fp). Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour MECA-79 anticorps (Biotin) (ABIN7656727)

Antigène: MECA-79

Reactivité: Humain, Souris, Rat

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Biotin

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MECA-79

Détail du produit

Fonction: MECA-79(MECA-79), Biotin conjugate

Attributs du produit: MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set of antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1, 3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with PNAd from mouse, human and many other species and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Mouse lymph node stromal cells

Isotype: IgM, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Positive Control: Human or mouse tonsil.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détail du antigène

Antigène: MECA-79

Sujet: MECA-79, Peripheral Node Addressin (PNAd)

Poids moléculaire: 90-110 kDa